SPLIT INTEINS PROTEIN MODIFICATION

Splittera is a tool for site-specific protein modification, both in vivo and in vitro.

The use of ZIP's split inteins can form a covalent bond between a protein and virtually any kind of molecule, including other proteins, fluorescent probes, peptides, DNA or a chemical compound.

Splittera applications in bioconjugation:

- Conjugation of a fluorescent probe to the N- or C-terminus of a protein

- Generation of bispecific antibodies by conjugating two different Fabs to the Fc portion of an antibody in a fast and simple reaction

- Generation of large libraries of cyclic peptides for drug discovery

- Generation of high molecular weight protein-based polymers