Splittera split inteins allow to site-specifically modify antibodies with toxins (Antibody drug conjugates, ADCs), fluorescent probes, DNA, peptides and proteins, rendering homogeneous products in a very efficient and cost-effective process.
Splittera for site-specific antibody modification:
– covalently incorporates payloads to the C-terminus of the heavy and light chains of an antibody, yielding DAR 2 or DAR 4 (DAR, drug to antibody ratio)
– the affinity of the antibody for the antigen is not affected
– allows to modify the antibody with two different molecules by using two orthogonal ZIP’s inteins, achieving DAR 2+2 in a single reaction
– it can be applied to any antibody format, including bispecific antibodies, Fabs, scFv and others.
A particular application of split-intein mediated bioconjugation is the generation of bispecific antibody formats, such as full-length IgGs, BiTEs, and other combinations including scFv domains or Fabs.
Orthogonal ZIP’s inteins allow you to rapidly screen a high number of different antigen recognition sites to find the best bispecific antibody for your target.
Learn more in our CLICK BIO flyer