Splittera split inteins allow to increase the sensitivity of a diagnostic assay.

The most common strategy to prepare a diagnostic test is to immobilize the analyte detecting molecule on a surface by adsorption. However, this method yields different orientations of the analyte detecting molecule, some of which block, totally or partially, the recognition of the analyte, reducing the sensitivity of the assay.

Splittera split inteins in diagnostics:

– allow to covalently bind any protein to a surface in a controlled and oriented manner

– all the molecules on the surface are able to recognize and bind to the analyte

– enable to standardize protein immobilization on a surface

– multiple analyte detection through the use of the four orthogonal split inteins owned by ZIP