Splittera is a tool for site-specific protein modification, both in vivo and in vitro.
The use of ZIP’s split inteins can form a covalent bond between a protein and virtually any kind of molecule, including other proteins, fluorescent probes, peptides, DNA or a chemical compound.
Splittera applications in bioconjugation:
– Conjugation of a fluorescent probe to the N- or C-terminus of a protein
– Generation of large libraries of cyclic peptides for drug discovery
– Protein cyclization to increase protein stability
– Generation of high molecular weight protein-based polymers
Learn more about protein cyclization and cyclic peptides in our CY-CLICK flyer