CLICK’N CUT™ for tag-less protein purification

CLICK’N CUT™ is a universal and robust tool to obtain tag-less proteins in a one-step process based on intein affinity. It is suitable for every step of product and process development: R&D, pre-clinical, clinical and industrial manufacturing.

How does CLICK’N CUT™ work?

    1. POI recombinantly produced in the host of choice fused to IntC.
    2. Resin, with IntN covalently linked to it, captures IntC-POI fusion thanks to the high affinity existing between the two intein domains.
    3. All undesired proteins are washed away.
    4. Intein mediated cleavage is triggered by a change in buffer’s pH.
    5. Pure tag-less protein elutes from the column
    6. The column can be easily regenerated.

KEY FEATURES of CLICK'N CUT™

  • Universal system to purify any POI
  • High purity (>95%)
  • Fast (5 hours to pure, tag-less POI)
  • Simple: cleavage is triggered on column by a pH shift at physiological conditions
  • Compatible with common expression host and buffers
  • Generates a clean cut with no extra residues
  • No premature cleavage during protein expression
  • Cleavage takes place regardless of the identity of the first POI’s amino acid

Learn more in our CLICK’N CUT flyer